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Here, we present an R-based pipeline for differential analyses of HDCyto data, largely based on Bioconductor packages. High dimensional mass and flow cytometry (HDCyto) experiments have become a method of choice for high throughput interrogation and characterization of cell populations. Institute for Molecular Life Sciences, University of Zurich, 8057 Zurich, Abstract Institute for Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerlandĭepartment of Experimental Oncology, European Institute of Oncology, Via Adamello 16, I-20139 Milan, Italyĭepartment of Dermatology, University Hospital Zurich, CH-8091 Zurich, Switzerland Institute of Experimental Immunology, University of Zurich, 8057 Zurich, Switzerland Institute for Molecular Life Sciences, University of Zurich, 8057 Zurich, Carsten Krieg CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets. Tuned softer than a MX engine, the Ossa grunted around the track fairly well. I saw a Pioneer race motocross out at Haney Town a long time ago. I felt the Ossa name was sullied for no good reason and if it was me that did something so stupid (and I have) I would have kept quiet about the situation. Even as a young whippersnapper I knew you couldn’t let stuff bounce around on a dirt bike. One road tester stuck a spare plug in the Ossa’s fiberglass rear fender storage area and then complained when the loose plug beat a hole in the compartment…after riding trails. The cycle magazines of that era praised the Ossa for its handling and generally good off road manners. So maybe rarity has something to do with my fascination with the brand. The Ossa was similar in construction to a Bultaco but where there were four or five Bultacos running around the town where I grew up there were no Ossas. (Don’t leave your weed whacker in the back of a pickup truck for 6 months.) I’m assuming that problem has been fixed because the IMS rotocast tank on the Husky has held up fine for 5 years. The new stuff is not without its problems as I’ve had fuel tanks that were exposed to sunlight crumble into dust. Rotocast, alcohol resistant, poly-some-such-crap has taken its place. Unfortunately, modern alcohol laced fuel plays hell with fiberglass so the use of the miracle plastic has faded. The Spanish built Ossa’s bodywork was fiberglass, the original miracle plastic that many manufacturers used back in the late 1960s and 1970s. That cool rear fender extension gave the bike a flat track look that I have been a sucker for since forever. It was like some sort of cheerful, horse drawn funeral wagon. I loved the old-fashioned, pin-striped black panels over the orange base. It does not store any personal data.The styling of the 250cc Ossa Pioneer is what attracted me to the bike in the first place. 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